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Software
Flow cytometer analysis on PC
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mlinde
Member
United States
11 Posts Posted - February 03 2006 : 6:21:13 PM
________________________________________
I'm looking into flow cytometer analysis programs for the PC and
I was
wondering whether anyone had any insight into which programs were the
most useful and worthwhile. From what I have seen, Flow-Jo and
FCSexpress are the more commonly used PC programs. Does anyone have
any experience with either of these programs? The other option would
be to purchase another Macintosh and use CellQuest. Advice, comments,
suggestions?
rakeshverma
Member
United States
93 Posts Posted - February 04 2006 : 10:46:54 PM
________________________________________
I have used CellQuest. Its a good option.
Antonio68
Member
Germany
20 Posts Posted - May 01 2006 : 11:48:43 AM
________________________________________
You can use Summit from Dako is free and gives you the possibility to
do offline compensation. However, the best flow cytometry software is
FlowJo. There is a PC (Java based) and a Mac version. The Mac version
for the moment is quite better.
lovesthelab *
Senior Member
United States
804 Posts Posted - July 06 2006 : 4:59:46 PM
________________________________________
I'm not a PC person, but am an ardent flow person. My flow friends
with PCs swear by WinMidi, which is free. If you Google for free flow
cytometry software, you'll come to 2 sites, one at UMass, one at
Scripps. Lots of free analysis software for the PC, maybe because BD
relied on Macs for so long.
mlinde
Member
United States
11 Posts Posted - August 02 2006 : 5:44:55 PM
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy with either of them compared to Cell Quest.
I found FCSexpress almost impossible to work with. FlowJo was
alright,

but I don't think I had the time to really get the software down.

Anyone know if BD ever plans to put out CellQuest on PC?

Lovesthelab*
Senior Member
United States
804 Posts Posted - August 09 2006 : 1:22:04 PM
________________________________________
quote:
________________________________________
As an update, I tried trial versions of FCSexpress and FlowJo and
wasn't really happy with either of them compared to Cell Quest. I
found FCSexpress almost impossible to work with. FlowJo was alright,
but I don't think I had the time to really get the software down.
Anyone know if BD ever plans to put out CellQuest on PC?
________________________________________
Don't quote me,
but
I do believe that CellQuest is available for PC
because the newer BD flow cytometers are digital and use PCs for
acquisition.
Mlinde, have you ever tried WinList or WinMidi?
Both of those were written for PCs. Also WEASEL comes in PC and Mac
versions.

Did you read the flow perspectives?
There is a software discussion
there.
(Disclosure-- I wrote it).

Mlinde or anyone else, you can contact me through the SAB e-mail
system
if you want to discuss flow software or anything flow some more
off the forum.

I'll be glad to help if I can.

Edited by – lovesthelab* on August 09 2006 1:24:07 PM
rgrant
Moderator
Australia
2350 Posts Posted - October 10 2007 : 02:34:47 AM
________________________________________
Well, it took me about
30 seconds to discover
that Mitchell Haynes is

VP Sales at Kanecki – see
http://www.kanecki.com/about.html

Admin, this is blatant advertising
(MH started this thread yesterday).
--
"I don't have a lot of use for Coldplay most of the time" -- rwintle
http://network.nature.com/blogs/user/rpg
rwintle
Advanced Member
Canada
4684 Posts Posted - October 10 2007 : 10:49:46 AM
________________________________________
AND SOMEBODY TELL HIM TO STOP SHOUTING.
khenwood67
Administrator
United States
247 Posts Posted - October 10 2007 : 11:03:53 AM
________________________________________
I've deleted the topic that he started, and also deleted his post in
this forum. He won't be posting on the forums again.
Kathryn Henwood
Membership Coordinator
The Science Advisory Board
k.henw...@scienceboard.net

On Aug 10, 3:55 pm, buybro...@yahoo.com wrote:
Re: mr on Apple web site
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From: J. Paul Robinson <jpr@flowcyt.cyto.purdue.edu>
Date: Mon Aug 23 2004 - 18:46:12 EST
I knew that I should not have taken Mario's bait....but its
been a long summer.....!
paul



J.Paul Robinson, PhD PH765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu

Have you seen our new HCS webpage?
http://www.cyto.purdue.edu/hcs
Received on Tue Aug 24 15:58:00 2004
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RE: mr on Apple web site
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From: Fischer, Randy (NIH/NIAMS) <fischer1@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,

I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we have
MACs
(and associated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.

Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.

Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.

Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fischer1@mail.nih.gov

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On Aug 11, 4:25*am, Mitch Haynes <mitchhay...@gmail.com> wrote:
Here is more information from the Purdue Cytometry mail list on
Facebook

http://www.facebook.com/note.php?not...9772873&ref=mf

On Aug 12, 8:38*pm, buybro...@yahoo.com wrote:

http://www.facebook.com/note.php?not...9772873&ref=mf
http://www.facebook.com/note.php?not...9772873&ref=mf

On Aug 10, 3:55*pm, buybro...@yahoo.com wrote:
OVER 400 POSTINGS FROM THE PURDUE CYTOMETRY MAIL LIST

http://www.facebook.com/note.php?not...9772873&ref=mf

http://www.facebook.com/note.php?not...9772873&ref=mf