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Product Reviews
Amnis ImageStream 100
Flow Cytometry Plus Microscopy
"The visualpower of microscopy plus the statistical rigor of flow
cytometry rolled into 1 package." That's the company's tag line. And
they deliver.
I'm a BD flow lover, a confirmed Mac user (I tell everyone "I don't do
Windows"). So why am I touting a machine made by Amnis that requires
Windows? Because this instrument goes above and beyond mere flow
cytometry- it shows you every event (that's cell to non-flow users) in
detail. I used it and I am sold. It takes a single experiment now to
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activation assay. What's not to love? Even the computer quandary is
solvable (the one feature I'd change: I'd have it run off a Mac) -
with an Intel Mac; just get Parallels software, install Windows, and
you are good to go, or rather, run. And the ImageStream can run off
an Intel Mac this way too. I bought an Intel iMac just for analysis.
Installation of Parallels was easy, IDEAS is about as intuitional as
any flow software package. And Amnnis reps bend over to be helpful.
Don't let the price tag scare you; rather, help write a shared-
instrument grant (we did) for your institution to get one. It is
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Review by lovesthelab
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Software
Calculation of fluorescence ratio using WinMDI 2.9
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Tobsi
Member
Austria
1 Posts Posted - May 07 2007 : 09:02:31 AM Show Profile Visit
Tobsi's Homepage Reply with Quote Reply to Topic Hello!
Can anyone help me with the following problem:
I have FACS data (FACS Aria / DIVA Software) and need to determine the
fluorescence ratio of two recorded fluorescences (eg. FITC/PE). What I
hope to get is the fluorescence ratios of each cell resulting in a new
histogram, in order to determine the mean fluorescence of the ratio
peak...
It should be possible with WinMDI as I read in a paper:
"Data analysis was performed afterwards
with WinMDI 2.8 software, version 1.0 (Purdue University, Cytometry
Laboratories
[http://facs.scripps.edu/software.html]). The ratio of fluorescence
emission
was calculated for every cell by dividing the emission signal at 585
nm by the
emission signal at 670 nm. For any sample, the mean pHi was calculated
from the
mean of the ratios of all cells by using the calibration described
below."
Unfortunatly I couldn't figure out how exactly it works. Hope anyone
can help me! Big thanx!!!
lovesthelab
Senior Member
United States
810 Posts Posted - May 07 2007 : 10:02:33 AM Show Profile Visit
lovesthelab's Homepage Reply with Quote Reply Although I don't use
WinMIDI, it seems to me that you should be able to calculate the
ratios as the ratios of the mean fluorescence intensities for the
samples. That is, if WinMIDI doesn't have a ratio function in the
menus. I use CellQuest Pro, WEASEL, and used to used WinList. What
paper is it?
Send me a PM and we can e-mail each other.
Also, are you on the Purdue flow e-mail user's list? It's a great
place for flow advice. See the perspectives article on flow listing
good resources (written by me, a little plug here).
been there, done that more times than I care to admitGo to Top of Page
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